of the idea:
master project aims at optimizing the polymer production in bacteria with a
view to increase the production yield and reduce the cost of
We have isolated bacterial strains
that are capable of producing biological polymers that are relevant for multiple
different purposes in the industry. Metabolic engineering will be used to increase
efficiency and yield during fermentation. A number of candidate genes are to be
cloned into a plasmid and analysed under a diverse set of fermentation
project is seeking a student with a strong interest in laboratory work, including
molecular cloning and microbiology. The project will include practical work
in a lab at DTU Food in Lyngby.
I. Use the Gibson cloning method to insert selected candidate
genes into a plasmid vector
II. Characterize both wild type and engineered clones with
respect to polymer production, bacterial growth rate, and under different fermentation
evolution to increase yield further.
Sequencing by NGS
V. Analyse the functionality of the produced polymers.
For more information contact:
Forskningsgruppen for Mikrobiel Bioteknologi
Danmarks Tekniske Universitet