In 2018, Novozymes together with Astra ran
the “Masseeksperimentet 2018”, where 25,000 Danish school students collected
samples of plants, tested for presence of lactic acid producing bacteria (LAB).
From 11142 samples submitted to Novozymes 3906 unique LAB isolates were
identified. Most of the strains belong to already known species of LAB, but
subset of these were identified by whole genome sequencing. Sequence data
indicate that these represent at least 10 novel species of LAB.
In collaboration with DTU food Novozymes
would like to characterize LAB strains of the novel specie to make a formal
description of these species. Formal description of novel bacteria requires a
publication in “International Journal of Systematic and Evolutionary
Microbiology”. The requirements for such publication is relatively well
described. The description of novel bacterial species is based on physiological
and biochemical characterization of the strains as well as data indication the
genomic distance to the closest related valid described bacterial species. The
formal genetic basis for species distinction was based on 70% DNA/DNA homology,
which when comparing whole genome sequencing translate to 95% pairwise ANI.
In this project, the novel isolates are
divided into taxa based on pairwise ANI analysis. This may be supplemented with
additional bioinformatic characterizations. The novel isolates are preserved in
a collection in a format suitable for conducting the various test in a semi-automated
manner. The assays for this need to be set up and validates using relevant
benchmark strains. (Type strains of the closest relatives).
Microbiology, Assays, Automation, Bioinformatics
I samarbejde medNovozymes