Projekt

Micro and Nanofabrication projects

Udbyder

Vejleder

Sted

København og omegn

In the Nanofluidics and Bioimaging group, we use micro and nanofluidics to facilitate the imaging of biological particles. We have previously worked with single cells [1], chromosomes [2] and DNA molecules [3,4].  Each new idea starts with the design and fabrication of a fluidic device follow by testing and application, often involving fluorescence microscopy.

Fabrication methods could be micro/nano fabrication in cleanroom, micro-milling of a metal master for injection moulding, 3D printing.

In one project, we want to make a microfluidic flow cells for imaging cells using advanced super-resolution microscopy. With access to a new type of optical super-resolution microscope, you can be the first one to design and fabricate a flow cell for that imaging technique with applications in biology and biophysics.

In another project, we want to make a device for creating solute and solvent gradients for the imaging of nanoparticles. This has application in biophysics.

You will design a device, perform simulations, fabricate your device and test it. You will be supervised for working with microfluidic device design, cleanroom fabrication, fluorescence microscopy and practical microfluidic work. If you already have worked with cleanroom microfabrication, this is a great way to kick start your project. Contact me to know more!

http://www.nanotech.dtu.dk/Research-mega/Forskningsgrupper/Nanofluidics-Bio

Reference:

1.Marie, R., Pødenphant, M., Koprowska, K., Baerlocher, L., Vulders, R. C. M., Wilding, J., … Mir, K. U. (2018). Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device. Lab on a Chip, 18(13), 1891–1902. https://doi.org/10.1039/c8lc00169c

2. Rasmussen, K. H., Marie, R., Moresco, J. L., Svendsen, W. E., Kristensen, A., & Mir, K. U. (2011). A device for extraction, manipulation and stretching of DNA from single human chromosomes. Lab on a Chip, 11(8), 1431–1433. https://doi.org/10.1039/c0lc00603c 

3. Marie, R., Pedersen, J. N., Mir, K. U., Bilenberg, B., & Kristensen, A. (2018). Concentrating and labeling genomic DNA in a nanofluidic array. Nanoscale, 10(3), 1376–1382. https://doi.org/10.1039/c7nr06016e

4. Marie, R., Pedersen, J. N., Bærlocher, L., Koprowska, K., Pødenphant, M., Sabatel, C., … Mir, K. U. (2018). Single-molecule DNA-mapping and whole-genome sequencing of individual cells. Proceedings of the National Academy of Sciences of the United States of America, 201804194. https://doi.org/10.1073/pnas.1804194115

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Kontakt

Virksomhed/organisation

DTU Sundhedsteknologi

Navn

Rodolphe Marie

Stilling

Gruppeleder, Lektor

Mail

rcwm@dtu.dk

Vejleder-info

Bachelor i Produktion og Konstruktion

Vejleder

Rodolphe Marie

ECTS-point

5 - 35

Type

Bachelorprojekt, Fagprojekt, Kandidatspeciale, Specialkursus

Kandidatuddannelsen i Konstruktion og Mekanik

Vejleder

Rodolphe Marie

ECTS-point

5 - 35

Type

Bachelorprojekt, Fagprojekt, Kandidatspeciale, Specialkursus

Bachelor i Fysik og Nanoteknologi

Vejleder

Rodolphe Marie

ECTS-point

5 - 35

Type

Bachelorprojekt, Fagprojekt, Kandidatspeciale, Specialkursus

Kandidatuddannelsen i Fysik og Nanoteknologi

Vejleder

Rodolphe Marie

ECTS-point

5 - 35

Type

Bachelorprojekt, Fagprojekt, Kandidatspeciale, Specialkursus

OM DTU

DTU er et teknisk eliteuniversitet med international rækkevidde og standard. Vores mission er at udvikle og nyttiggøre naturvidenskab og teknisk videnskab til gavn for samfundet. 10.000 studerende uddanner sig her til fremtiden, og 5.700 medarbejdere har hver dag fokus på uddannelse, forskning, myndighedsrådgivning og innovation, som bidrager til øget vækst og velfærd.

Find os her

Anker Engelunds Vej 1
Bygning 101A
2800 Kgs. Lyngby


45 25 25 25

dtu@dtu.dk

CVR-nr. 30 06 09 46

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