Projekt

Gut health product discovery with microfluidics

Udbyder

Vejleder

Sted

København og omegn

Project outline

Fermentable Oligo, Di, Mono-saccharides and Polyols are short chain carbohydrates that are good carbon sources for bacteria such as Lactic acid bacteria. Lactic acid bacteria are important for various applications within food for fermentation of milk into yoghurt, cheese etc., for fermentation of vegetables, fish and meat for conservation eg. dry sausages like salami. Also, for many years lactic acid bacteria have been used as food supplement for various health reason to balance the human and animal gut. Especially useful to treat diarrhea or more recently also to secure the health of small children from bacterial infections which under certain conditions in poor and malnourished children otherwise could lead to sepsis and in some of these cases death. [1].

In 2016, Novozymes together with Astra ran the Masseeksperimentet 2018, where 25000 Danish school students went out into nature to isolate new Lactobacillus strains, finding 3906 unique isolates of which 649 are unknown bacterial strains [2]. Now we would like to characterize the carbon source preferences of this large set of new microbial diversity. To make this characterization ultra-fast our microfluidics technology will be used. This lab-on-the-chip technology allows the targeted description of millions of microbes in just a few hours [3].

During the project the student will design growth media that allows optimal growth of Lactobacilli. The microbes are grown and selected with the microfluidics technology and further characterized with Biotyper and next generation sequencing (NGS).

Technologies: Microfluidics, Lactobacillus cultivation, NGS, BioTyper

References

[1] Panigrahi, Pinaki & Parida, Sailajanandan & C. Nanda, Nimai & Satpathy, Radhanath & Pradhan, Lingaraj & Chandel, Dinesh & Baccaglini, Lorena & Mohapatra, Arjit & S. Mohapatra, Subhranshu & R. Misra, Pravas & Chaudhry, Rama & H. Chen, Hegang & Johnson, Judith & Glenn Morris, J & Paneth, Nigel & Gewolb, Ira. (2017). A randomized synbiotic trial to prevent sepsis among infants in rural India. Nature. 548. 10.1038/nature23480.

[2] https://naturvidenskabsfestival.dk/masseeksperiment

[3] Lu, Heng, et al. "High throughput single cell counting in droplet-based microfluidics." Scientific Reports 7.1 (2017): 1366.

I samarbejde med

Novozymes

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Kontakt

Virksomhed/organisation

DTU Fødevareinstituttet

Navn

Claus Heiner Bang-Berthelsen

Stilling

Seniorforsker

Mail

claban@food.dtu.dk

Vejleder-info

Kandidatuddannelsen i Bioteknologi

Vejleder

Claus Heiner Bang-Berthelsen

ECTS-point

25 - 30

Type

Kandidatspeciale

Kandidatuddannelsen i Kemisk og Biokemisk Teknologi

Vejleder

Claus Heiner Bang-Berthelsen

ECTS-point

25 - 30

Type

Kandidatspeciale

OM DTU

DTU er et teknisk eliteuniversitet med international rækkevidde og standard. Vores mission er at udvikle og nyttiggøre naturvidenskab og teknisk videnskab til gavn for samfundet. 10.000 studerende uddanner sig her til fremtiden, og 5.700 medarbejdere har hver dag fokus på uddannelse, forskning, myndighedsrådgivning og innovation, som bidrager til øget vækst og velfærd.

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Anker Engelunds Vej 1
Bygning 101A
2800 Kgs. Lyngby


45 25 25 25

dtu@dtu.dk

CVR-nr. 30 06 09 46

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