is commonly used in food fermentations. Important traits of industrially important L. lactis
strains are located on plasmids. Plasmids seems to be important for the evolution and adaption of L. lactis
to the dairy environment. Plasmids are generally very important for evolution of procariots.
In this project the genome sequences of isolates of L. lactis should be analyzed for the presence of replication proteins and plasmid replication origins. Replicons will be isolated by PCR and furnished with a selectable marker by Gibson assembley to create selectable plasmids. Compatibility and copy number of the constructed plasmids will be characterized.
Interest for and experience with molecular biology