Nanofluidics is a technology that
enables to array and manipulate single molecules and supramolecular assemblies
like lipid vesicles. In experiments, nanofluidics is combined with fluorescence
microscopy. Recently we worked with genomic DNA, as a physical object [1,2] but
also as the carrier for the genetic code . Working with experimental nanofluidics
gives the opportunity to investigate biophysics and/or pursue technological
goals with applications in life science.
This project is an
interdisciplinary project based on experimental work with nanofluidics devices
and single molecule fluorescence microscopy. In particular, you will start
doing simple experiments to get trained in microscopy and nanofluidics using
existing nanofluidics devices. From there, the project is very open and you
will define your experimental work and hypothesis with your supervisor. You
will be supervised in working with single molecule fluorescence microscopy,
micro and nanofluidics experimental work and data analysis based on image
1. Pedersen, J. N., Marie, R., Kristensen,
A., & Flyvbjerg, H. (2016). How to determine local stretching and tension
in a flow-stretched DNA molecule. Physical Review E, 93(4), 042405.
2. Pedersen, J. N., Lüscher, C. J.,
Marie, R., Thamdrup, L. H., Kristensen, A., & Flyvbjerg, H. (2014).
Thermophoretic forces on DNA measured with a single-molecule spring balance.
Physical Review Letters, 113(26), 268301
3. Marie, R.,
Pedersen, J. N., L. V. Bauer, D., Rasmussen, K. H., Yusuf, M., Volpi, E., … U.
Mirb, K. (2013). Integrated view of genome structure and sequence of a single
DNA molecule in a nanofluidic device. National Academy of Sciences.
Proceedings, 110(13), 4893–4898