Purpose of the idea:
project will elucidate an alternative road to oral peptide therapy exemplified
with hormones relevant in human medicine delivered by lactic acid bacteria
We have developed
LAB peptide delivery platform which is highly efficient compared with current
bacterial drug delivery vectors based on LAB. The student will work with different
strains of LAB including our platform strain.
project will include a comparison of yields between different strains as well
as engineering of these for optimised production of peptides with relevant
medical purposes for treatment of different metabolic and inflammatory diseases.
The project is seeking a student with a strong interest for laboratory work as
well as an interest in biomedical science and microbiology.
We are collaborating with other groups at DTU Food and we have the
possibility to conduct animal experiments with promising bacterial strains.
I. Initial candidates are cloned into the bacterial
strain by the use of Gibson cloning vector including different tags for various
II. Characterize both wild type and mutant clones of L. Lactis with respect to protein secretion
yield and activity in vitro.
Analyse how the
strains and peptides tolerate the environment in the intestines (by setting up ex vivo fermentation in a gastric micro environment).
Analyse to what
extent efficient protein production yield and fast growth can be obtained under
For more information contact:
Forskningsgruppen for Mikrobiel
Bioteknologi og Bioraffinering
Danmarks Tekniske Universitet