Project

Multiphoton Fluorescence Lightsheet Microscopy

Publisher

Supervisor

Location

Greater Copenhagen area

Lightsheet Microscopy is a technique where a sample is illuminated with a sheet of light perpendicularly to the direction of observation. A whole slice of the sample can be recorded with a camera chip, enabling fast optical sectioning. At the same time, photostress or photodamage dealt to the sample are reduced, since out of focus parts of the samples are not illuminated.

Multiphoton Fluorescence Microscopy uses two or more photons in the infrared part of the spectrum to excite a fluorophore. This enables larger imaging depths in scattering media, due to reduced scattering and, in biological tissue, less absorption. It also facilitates separation of excitation and emission light, due to their large difference in wavelength.

Together with the University of St. Andrews, we aim to further advance the combination of the two, multiphoton lightsheet microscopy. St. Andrews has a system in place. We want to add and test our diode based laser source to that system, and bring back the knowledge to DTU and build a similar system ourselves.

Part of this project is conducted at the University of St. Andrews, where the student gets trained in lightsheet microscopy. The student will bring one of our laser sources to St. Andrews and implement it into the existing setup. The student will then bring back the knowledge about the setup and build a multiphoton lightsheet microscope in our own labs.

In collaboration with

University of St. Andrews

Requirements

Hands-on experimental experience, Knowledge on optics

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Contact

Company / Organization

DTU Sundhedsteknologi

Name

Dominik Marti

Position

Forsker

Mail

domar@dtu.dk

Supervisor info

MSc in Physics and Nanotechnology

Supervisor

Dominik Marti

Co-supervisors

Peter E. Andersen

ECTS credits

30 - 35

Type

MSc thesis

MSc in Engineering, Photonics Engineering

Supervisor

Dominik Marti

Co-supervisors

Peter E. Andersen

ECTS credits

30 - 35

Type

MSc thesis

MSc in Biomedical Engineering

Supervisor

Dominik Marti

Co-supervisors

Peter E. Andersen

ECTS credits

30 - 35

Type

MSc thesis

Technical University of Denmark

For almost two centuries DTU, Technical University of Denmark, has been dedicated to fulfilling the vision of H.C. Ørsted – the father of electromagnetism – who founded the university in 1829 to develop and create value using the natural sciences and the technical sciences to benefit society.


Today, DTU is ranked as one of the foremost technical universities in Europe, continues to set new records in the number of publications, and persistently increases and develops our partnerships with industry, and assignments accomplished by DTU’s public sector consultancy.

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Denmark



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