Lightsheet Microscopy is a technique where a
sample is illuminated with a sheet of light perpendicularly to the direction of
observation. A whole slice of the sample can be recorded with a camera chip,
enabling fast optical sectioning. At the same time, photostress or photodamage dealt
to the sample are reduced, since out of focus parts of the samples are not
Multiphoton Fluorescence Microscopy uses
two or more photons in the infrared part of the spectrum to excite a
fluorophore. This enables larger imaging depths in scattering media, due to
reduced scattering and, in biological tissue, less absorption. It also
facilitates separation of excitation and emission light, due to their large
difference in wavelength.
Together with the University of St.
Andrews, we aim to further advance the combination of the two, multiphoton
lightsheet microscopy. St. Andrews has a system in place. We want to add and
test our diode based laser source to that system, and bring back the knowledge
to DTU and build a similar system ourselves.
of this project is conducted at the University of St. Andrews, where the
student gets trained in lightsheet microscopy. The student will bring one of
our laser sources to St. Andrews and implement it into the existing setup. The
student will then bring back the knowledge about the setup and build a
multiphoton lightsheet microscope in our own labs.
In collaboration withUniversity of St. Andrews
Hands-on experimental experience, Knowledge on optics