Project

Taxonomic characterization of novel lactic acid producing bacteria

Publisher

Supervisor

Location

Greater Copenhagen area

Project outline

In 2018, Novozymes together with Astra ran the “Masseeksperimentet 2018”, where 25,000 Danish school students collected samples of plants, tested for presence of lactic acid producing bacteria (LAB). From 11142 samples submitted to Novozymes 3906 unique LAB isolates were identified. Most of the strains belong to already known species of LAB, but subset of these were identified by whole genome sequencing. Sequence data indicate that these represent at least 10 novel species of LAB.

In collaboration with DTU food Novozymes would like to characterize LAB strains of the novel specie to make a formal description of these species. Formal description of novel bacteria requires a publication in “International Journal of Systematic and Evolutionary Microbiology”. The requirements for such publication is relatively well described. The description of novel bacterial species is based on physiological and biochemical characterization of the strains as well as data indication the genomic distance to the closest related valid described bacterial species. The formal genetic basis for species distinction was based on 70% DNA/DNA homology, which when comparing whole genome sequencing translate to 95% pairwise ANI.

In this project, the novel isolates are divided into taxa based on pairwise ANI analysis. This may be supplemented with additional bioinformatic characterizations. The novel isolates are preserved in a collection in a format suitable for conducting the various test in a semi-automated manner. The assays for this need to be set up and validates using relevant benchmark strains. (Type strains of the closest relatives).

 

 Technologies: Microbiology, Assays, Automation, Bioinformatics

 

In collaboration with

Novozymes

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Contact

Company / Organization

DTU Fødevareinstituttet

Name

Claus Heiner Bang-Berthelsen

Position

Seniorforsker

Mail

claban@food.dtu.dk

Supervisor info

MSc in Bioinformatics and Systems Biology

Supervisor

Claus Heiner Bang-Berthelsen

ECTS credits

25 - 30

Type

MSc thesis

MSc in Biotechnology

Supervisor

Claus Heiner Bang-Berthelsen

ECTS credits

25 - 30

Type

MSc thesis

MSc in Food Technology

Supervisor

Claus Heiner Bang-Berthelsen

ECTS credits

25 - 30

Type

MSc thesis

MSc in Chemical and Biochemical Engineering

Supervisor

Claus Heiner Bang-Berthelsen

ECTS credits

25 - 30

Type

MSc thesis

Technical University of Denmark

For almost two centuries DTU, Technical University of Denmark, has been dedicated to fulfilling the vision of H.C. Ørsted – the father of electromagnetism – who founded the university in 1829 to develop and create value using the natural sciences and the technical sciences to benefit society.


Today, DTU is ranked as one of the foremost technical universities in Europe, continues to set new records in the number of publications, and persistently increases and develops our partnerships with industry, and assignments accomplished by DTU’s public sector consultancy.

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