Project

Micro and Nanofabrication projects

Publisher

Supervisor

Location

Greater Copenhagen area

In the Nanofluidics and Bioimaging group, we use micro and nanofluidics to facilitate the imaging of biological particles. We have previously worked with single cells [1], chromosomes [2] and DNA molecules [3,4].  Each new idea starts with the design and fabrication of a fluidic device follow by testing and application, often involving fluorescence microscopy.

Fabrication methods could be micro/nano fabrication in cleanroom, micro-milling of a metal master for injection moulding, 3D printing.

In one project, we want to make a microfluidic flow cells for imaging cells using advanced super-resolution microscopy. With access to a new type of optical super-resolution microscope, you can be the first one to design and fabricate a flow cell for that imaging technique with applications in biology and biophysics.

In another project, we want to make a device for creating solute and solvent gradients for the imaging of nanoparticles. This has application in biophysics.

You will design a device, perform simulations, fabricate your device and test it. You will be supervised for working with microfluidic device design, cleanroom fabrication, fluorescence microscopy and practical microfluidic work. If you already have worked with cleanroom microfabrication, this is a great way to kick start your project. Contact me to know more!

http://www.nanotech.dtu.dk/Research-mega/Forskningsgrupper/Nanofluidics-Bio

Reference:

1.Marie, R., Pødenphant, M., Koprowska, K., Baerlocher, L., Vulders, R. C. M., Wilding, J., … Mir, K. U. (2018). Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device. Lab on a Chip, 18(13), 1891–1902. https://doi.org/10.1039/c8lc00169c

2. Rasmussen, K. H., Marie, R., Moresco, J. L., Svendsen, W. E., Kristensen, A., & Mir, K. U. (2011). A device for extraction, manipulation and stretching of DNA from single human chromosomes. Lab on a Chip, 11(8), 1431–1433. https://doi.org/10.1039/c0lc00603c 

3. Marie, R., Pedersen, J. N., Mir, K. U., Bilenberg, B., & Kristensen, A. (2018). Concentrating and labeling genomic DNA in a nanofluidic array. Nanoscale, 10(3), 1376–1382. https://doi.org/10.1039/c7nr06016e

4. Marie, R., Pedersen, J. N., Bærlocher, L., Koprowska, K., Pødenphant, M., Sabatel, C., … Mir, K. U. (2018). Single-molecule DNA-mapping and whole-genome sequencing of individual cells. Proceedings of the National Academy of Sciences of the United States of America, 201804194. https://doi.org/10.1073/pnas.1804194115

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Contact

Company / Organization

DTU Sundhedsteknologi

Name

Rodolphe Marie

Position

Gruppeleder, Lektor

Mail

rcwm@dtu.dk

Supervisor info

BSc in Mechanical Engineering

Supervisor

Rodolphe Marie

ECTS credits

5 - 35

Type

BSc project, Course project, MSc thesis, Special course

MSc in Engineering, Mechanical Engineering

Supervisor

Rodolphe Marie

ECTS credits

5 - 35

Type

BSc project, Course project, MSc thesis, Special course

BSc in Physics and Nanotechnology

Supervisor

Rodolphe Marie

ECTS credits

5 - 35

Type

BSc project, Course project, MSc thesis, Special course

MSc in Physics and Nanotechnology

Supervisor

Rodolphe Marie

ECTS credits

5 - 35

Type

BSc project, Course project, MSc thesis, Special course

Technical University of Denmark

For almost two centuries DTU, Technical University of Denmark, has been dedicated to fulfilling the vision of H.C. Ørsted – the father of electromagnetism – who founded the university in 1829 to develop and create value using the natural sciences and the technical sciences to benefit society.


Today, DTU is ranked as one of the foremost technical universities in Europe, continues to set new records in the number of publications, and persistently increases and develops our partnerships with industry, and assignments accomplished by DTU’s public sector consultancy.

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