Fermentable Oligo, Di, Mono-saccharides and Polyols are short chain carbohydrates that are good
carbon sources for bacteria such as Lactic acid bacteria. Lactic acid bacteria
are important for various applications within food for fermentation of milk
into yoghurt, cheese etc., for fermentation of vegetables, fish and meat for
conservation eg. dry sausages like salami. Also, for many years lactic acid
bacteria have been used as food supplement for various health reason to balance
the human and animal gut. Especially useful to treat diarrhea or more recently
also to secure the health of small children from bacterial infections which
under certain conditions in poor and malnourished children otherwise could lead
to sepsis and in some of these cases death. .
In 2016, Novozymes together with Astra ran
the Masseeksperimentet 2018, where 25000 Danish school
students went out into nature to isolate new Lactobacillus strains, finding
3906 unique isolates of which 649 are unknown bacterial strains . Now we
would like to characterize the carbon source preferences of this large set of new
microbial diversity. To make this characterization ultra-fast our microfluidics
technology will be used. This lab-on-the-chip technology allows the targeted
description of millions of microbes in just a few hours .
During the project the student will design
growth media that allows optimal growth of Lactobacilli. The microbes are grown
and selected with the microfluidics technology and further characterized with
Biotyper and next generation sequencing (NGS).
Technologies: Microfluidics, Lactobacillus cultivation, NGS,
 Panigrahi, Pinaki & Parida,
Sailajanandan & C. Nanda, Nimai & Satpathy, Radhanath & Pradhan,
Lingaraj & Chandel, Dinesh & Baccaglini, Lorena & Mohapatra, Arjit
& S. Mohapatra, Subhranshu & R. Misra, Pravas & Chaudhry, Rama
& H. Chen, Hegang & Johnson, Judith & Glenn Morris, J & Paneth,
Nigel & Gewolb, Ira. (2017). A randomized synbiotic trial to prevent sepsis
among infants in rural India. Nature. 548. 10.1038/nature23480.
 Lu, Heng, et al. "High
throughput single cell counting in droplet-based microfluidics." Scientific
Reports 7.1 (2017): 1366.
In collaboration withNovozymes