Project

A native-like ex vivo transport model for oral-drug delivery

Publisher

Supervisor

Location

Greater Copenhagen area

Numerous systems and strategies have been progressed over the years to develop in vitro models that mimic the natural drug-barriers in the body. However, the highly restrictive gut-barrier still remains among the first obstacles for orally ingested drugs. The key characteristic of gut-barrier is the dense epithelial cell layer that forms a complex surface topology. The tight-junctions between these epithelial cells are instrumental in regulating the function of barrier, while the complex surface topology maximizes the surface area for selective absorption of compounds into the bloodstream. In this project, we aim to generate a native-like 3D model which is capable to be used for drug screening, and mimics the characteristics of native barrier. To this end, various topologies will be 3D printed using highly automated stereolithography, followed by human Caco-2 epithelial cells being cultured on the printed structures, in order to mimic the human gut-barrier in a more realistic fashion. 

In this study plan we aim to characterize the in vitro models and compare their functionality, integrity and permeability through the following plan:

 

·      Making different geometries on the culture surface using 3D printing.

·      human Caco-2 epithelial cells on these different topologies

·      Measuring transepithelial electrical resistance (TEER) in the systems over time

·      Immunostaining against different markers and confocal microscopy to further confirm cell differentiation and presence of transformers.

·      Testing the barrier integrity using a model molecule.

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Contact

Company / Organization

DTU Sundhedsteknologi

Name

Alireza Dolatshahi-Pirouz

Position

Gruppeleder, Adjunkt

Mail

aldo@dtu.dk

Supervisor info

BSc in Biomedical Engineering

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

BSc in Biotechnology

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

BSc in Human Life Science Engineering

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

BSc in Quantitative biology and disease modelling

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

Bachelor of Engineering, Chemical and Bio Engineering (prev. Chemical and Biochemical Engineering)

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

MSc in Biomedical Engineering

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

MSc in Biotechnology

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

MSc in Chemical and Biochemical Engineering

Supervisor

Alireza Dolatshahi-Pirouz

ECTS credits

2.5 - 30

Type

Final project, Other, BSc project, Course project, MSc thesis, Course-related projects, Special course

Technical University of Denmark

For almost two centuries DTU, Technical University of Denmark, has been dedicated to fulfilling the vision of H.C. Ørsted – the father of electromagnetism – who founded the university in 1829 to develop and create value using the natural sciences and the technical sciences to benefit society.


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